π Botany frontiers β 2026
What plant scientists are working on now. Each card states the open question, the evidence so far and the methods used β not results that have not happened yet.
Cβ rice
Open question: Can rice be re-engineered to use Cβ photosynthesis like maize, raising yield with less water and nitrogen?
Where it stands: International consortium since 2008; key Cβ genes and parts of the leaf anatomy have been introduced into rice, but a working Cβ cycle has not yet been achieved.
Builds on: HatchβSlack Cβ pathway (1966).
Synthetic biologyLeaf anatomyGene stackingRoot microbiome
Open question: Which soil microbes help roots take up nutrients or resist disease, and can we design that community?
Where it stands: Microbial inoculants are sold, but their field effects vary; research maps rootβmicrobe signalling.
Builds on: Mycorrhizal partnerships and strigolactone signalling.
MetagenomicsSynthetic communitiesField trialsGene-edited crops
Open question: How fast can CRISPR deliver hardier, more nutritious crops β and how should they be regulated?
Where it stands: Edited crops are on sale (e.g. high-GABA tomato in Japan, 2021). Several countries now regulate simple edits more lightly than GMOs.
Builds on: CRISPR in plants (2013).
CRISPR base & prime editingRegulationHeat & drought
Open question: How do plants sense heat and drought, and can crops be bred to keep yields in a warmer climate?
Where it stands: Heat sensors (e.g. phytochrome B acting as a thermosensor, 2016) found; breeding uses these genes.
Builds on: Plant hormone and light-signalling research.
Phenotyping dronesGenomicsSpeed breedingPlant communication
Open question: Do plants warn each other β through airborne chemicals or fungal networks?
Where it stands: Airborne signals between plants are well supported. Claims of a βwood-wide webβ sharing resources between trees are now disputed (review: Karst et al., 2023).
Builds on: Mycorrhizal networks.
Isotope tracingVolatile chemistryNitrogen-fixing cereals
Open question: Can wheat or maize fix their own nitrogen like beans, cutting fertiliser use?
Where it stands: Engineered microbes that fix nitrogen near cereal roots are being trialled; transferring nodule-forming ability is a longer-term goal.
Builds on: Legumeβrhizobia symbiosis.
Synthetic biologySymbiosis genetics